At present, there are still many problems in the helicopter power supply testing，such as multiple test items, complex test equipment, long test period and so on. If virtual instrument technology is applied to the design of the helicopter power supply test system, it would improve the test efficiency and shorten the test period. The test system designed in this paper use the PLC to control the switch of power supply circuits and test programs, use the signal conditioning circuits and PXI devices to acquire data, use the software developed on the CVI platform to analysis and display the data dynamically. In addition, relevant assessment strategies are used for the evaluation of specified performance and comprehensive performance. The test system can provide technical support and scientific basis for the use and maintenance of the helicopter power system, possessing a good application value.