In the traditional data acquisition system of LabVIEW, the real time performance of serial communication is not high and the efficiency of program run is to be improved. Aimed at the abovementioned present situation, one scheme of data acquisition system design of LabVIEW, which is based on the technology of MSComm and queue, is proposed. Wherein, DSP is the control core of slave computer to realize the data acquisition of analog front end and PC is a master computer. On the base of LabVIEW 2012, the application software of master computer is developed. Processing, storage, display and statistical analysis of sampled data of analog front end could be achieved. The testing results show that this design scheme is with the advantages of stable and reliable operation, simple and easyuse hardware interface, simple software program, easy maintenance and extension.