DICOM was an international standard for medical images and related information, it defined medical image formats that can be used for data exchange to meet clinical needs. In order to solve the problems in reading and displaying of the DICOM files, this article not only used classes and functions in ITK toolkit, VTK toolkit to read and display DICOM headers and images, but also used Qt flexible interface features to provide users with a friendly interaction Interface. According to the introduction of principle and methods of the toolkit reading and displaying the DICOM files, the article analyzed their advantages and disadvantages and the importance of mixed programming. Using the three mixed programming, we can achieve the DICOM image and header files batch, fast, correct read and display, but also make a preliminary exploration on the software development in this three integrated environment.