The inertial stabilization performance of aerial photoelectric platform is difficult to further improve only with classical Proportion Integration (PI) correction. The paper analyzed the main factors that affect the stabilization precision of the system, and designed an aerial photoelectric platform stabilization control algorithm based on a simplified disturbance observer (DOB). The algorithm is composed of DOB and PI controller, the DOB adopts gyroscope as feedback, utilizes the equivalent transform to achieve and without calculates the inverse model of the controlled system. Furthermore, an aerial photoelectric platform stabilization performance evaluation method based on isolation degree test is proposed. Finally, the inertial stabilization experiment is conducted in actual system, and the proposed stabilization control algorithm reached 10 μrad (RMS) stabilization precision, and also the isolation degree test results with new algorithm under 30 Hz band attitude disturbance has an obviously improvement than the conventional PI control.