For the actual demand of multiserial port, a USB2.0  based multichannel serial data acquisition scheme was proposed in this paper according to the characteristics of USB2.0 and serial transmission speed difference. In the respect of hardware, for the hot stuck equipment power supply restrictions and system reliability requirements, the main chip demonstration selection, and the system framework was designed; In the respect of software, in order to avoid multiserial active at the same time ‘preemptive’ USB interface and data frames loss, the multilevel cache structure was introduced to change the initiative to ‘preemptive’ for the passive stack. At the same time, the serial image stabilization capture model was designed by statistical method. In order to verify the effectiveness of the design model, the test scheme of 4way serial transmission was designed with the Demo platform as the test object. The test results meet the expected requirements and achieve the purpose of multichannel serial transmission.